Use of DREADDs to Dissect Metabolic Pathways

Nice review by Jurgen Wess here

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CNO in drinking water

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Considerations for immunofluorescence for various DREADD constructs

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I receive frequent queries regarding the appropriate way to visualize DREADDs.  Below I show the three basic DREADD constructs: HA-tagged mCherry fusion proteins HA-tagged in a vector with IRES-mCitrene In unfixed tissue the mCherry and mCitrene can be visualized using the parameters from the table below or by going here . NOTE: only the mCherry fusion protein fluorescence will show that the DREADD receptor is expressed.  mCitrene simply will show which cells were transfected or transduced. For visualizing HA we have a detailed recommended protocol   the principle of which is below. I also show various secondary antibodies which might be used along with excitation/emission characteristics Here's what you might consider if you have an mCherry fusion protein: Finally, what if you are using one of the HA-hM3Dq-IRES-mCitrene or HA-hM4Di-IRES-mCitrene constructs. ...
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Floxed DREADD mice now available from JAX

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