Showing posts from March, 2014

CNO via minipump

I've received several queries regarding the delivery of CNO via Alzet (or similar) minipumps.

As far as I'm aware no data using this as a delivery platform for CNO has been published (nor has my lab attempted this as we typically simply put CNO in the drinking water (

Nonetheless--it should work.  Anyone with success/failure feel free to add comments.

CNO in drinking water

We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies.

Here is our protocol:

1. Dissolve the CNO in a small volume of DMSO
2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml)
3. Give mice with CNO drinking water  and protect from light using foil-wrapped bottles
4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day)
5. Prepare fresh daily.
6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO