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Showing posts from September, 2016

AAV's now available from ADDGENE

These are exceptionally high quality with exceptional quality control and have been validated by Rothlab.

We launched the first 2 AAV on our website:  http://www.addgene.org/44361/ http://www.addgene.org/44362/
We're hoping the release the non DIO versions and control vector next week!

DREADD AAV's available in EU

the Viral Vector Facility (VVF) - an service facility of the Neuroscience Center Zurich, a joint network of more than 1000 researchers of the University of Zurich and ETH Zurich - as a source for DREADD AAV vectors (at least for European-based researchers, which makes processing probably more convenient for them)?

They will also  make small aliquots (50 ul) of various DREADD AAV vectors available through our Viral Vector Repository.

Contact information:

Jean-Charles Paterna, PhD Manager of the Viral Vector Facility Neuroscience Center Zurich University of Zurich and ETH Zurich Winterthurerstrasse 190 CH-8057 Zurich http://www.vvf.uzh.ch/en.html + 41 44 635 59 91

CELL: Opto/DREADDs/GcAMP/Rabies--what controls micturation behavior in mice

DREADD AAV's available via Duke Vector core

For further information contact:

Boris Kantor, PhD
Assistant Research Professor & Viral Vector Core Director Duke Department of Neurobiology-School of Medicine
412 Research Drive
Bryan Research Bldg room 213
Durham, NC 27710
Phone: 919-6811068

Update on AAVs for DREADDs

UNC is no longer offering single-unit AAV for DREADDs although we expect that these will be available via ADDGENE shortly.

To get onto the ADDGENE notification list contact:  Leila Haery haery@addgene.org with serotype and genotype needs for DREADD constructs. She will be helping Addgene coordinate production and distribution of DREADD viruses.


In the meantime, you can order plasmids from ADDGENE and simply ask UNC to make a custom prep for you or send the plasmids to the Penn Vector core.

Alternatively it looks like the Duke vector core is also offering DREADD-based AAVs.

Bryan

DREADD/Opto to identify VTA dopaminergic neurons which regulate ethologically relevant sleep–wake behaviors

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Very interesting paper found here.