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Showing posts from March, 2015

DREADDs vs OPTO: does it matter to the neuron?

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I get this question a lot and certainly there are a number of distinctions one can make with regard to these two technologies (Table 1).

TABLE 1. COMPARISON OF CHEMO- AND OPTOGENETIC PLATFORMS

Note to self: verify Cre-line before using

I received an email the other day suggesting that some AAV-based DREADDs were not working 'as advertised'.

To make a long email saga short it turns out that the Cre-driver line used had not been validated in the particular tissue being used.

Thus the reason the DREADD was not being expressed is because there was no expression of Cre in the cells of interest.

Here's what my lab does (and what we recommend) before using a Cre-line:

Check the literature for expression in the tissue/cell type of interestCheck the literature to see if any lab has ever used it to express a FLEX/DIO-based cargo in the cell type of interestIf not, use a AAV-FLEX-XFP virus and do a control experiment to see if the experiment will work as advertisedAlternatively use an Rosa6-FLOXED XFP reporter mouseIf the controls do not work this would indicate:The Cre-line does not express sufficient Cre in the cell of interestThere is something wrong with your technique Only after you have validated the Cre-line…

Webinar on DREADD2.0 available on YouTube

For those who missed the webinar it is available here.

Webinar on DREADD2.0

I will give a webinar on DREADD2.0 (updates and enhancements to DREADD technology) Wednesday (3/18/2015; 9 am PT).  As in the past the presentation will be archived and available on Youtube.

If you wish to see this in 'real time' go to the link above and register (it is free);  Q&A available during the talk.

Using DREADDs to deconstruct 'binge drinking'

Nice paper in Nature Neuroscience from Kash lab and colleagues--a lot of stuff here going from mice to primates.

"The DREADDed lightness of the future" with regard to seizure control

A nice review on the use of chemo- and optogenetic technologies for seizure control in Nature Neuroscience.