AAV's now available from ADDGENE

These are exceptionally high quality with exceptional quality control and have been validated by Rothlab.

We launched the first 2 AAV on our website: 

We're hoping the release the non DIO versions and control vector next week!

Comments

  1. Is there a particular cre-expressing virus which works best with these?

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  2. Any Cre-expressing virus which has been validated should work fine

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  3. Hi, Dr. Roth! I had checked the virus titer on the addgene website (≥ 2×10¹² vg/mL), it seems it's less than previous UNC production (5.7x10¹² vg/mL). Have you tested the virus in vivo? Thank you!

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  4. Dear Dr. Roth,
    If these viruses are injected into a Cre-line mouse, would they be expressed in the tagged neurons only? If not, do you recommend other viruses?
    Thank you

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    Replies
    1. They mention these viruses are "Cre-dependent", so does this answer "yes" to my question?
      Also, there are different serotypes, do you recommend one versus the other?

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    2. Yes. I typically recommend starting with AAV8

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    3. Sorry to keep asking, but is there a specific reason for choosing this one over 2 for example? ( Note: I work on the olfactory bulb which is a pretty large area in case this changes things)

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    4. No--we find it simply works nearly every time. You may wish to order several serotypes and simply test them side-by-side.

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  5. The DIO versions will only be expressed in cells expressing Cre-recombinase--so yes

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  6. Hi
    Is someone familiar with the technique of Clozapine administration via drinking water or osmotic mini-pump's ?

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