DREADD users blog

I've received several queries regarding the delivery of CNO via Alzet (or similar) minipumps. As far as I'm aware no data using this as a delivery platform for CNO has been published (nor has my lab attempted this as we typically simply put CNO in the drinking water ( http://chemogenetic.blogspot.com/2014/03/cno-in-drinking-water.html ) Nonetheless--it should work.  Anyone with success/failure feel free to add comments.

We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water  and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO