CNO in drinking water
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
Are there other mCitrine based variants of DREADDs such as AAV-hSyn-DIO-hm3D(Gq)-mcitrine available and validated?
ReplyDeleteWe recently performed a control experiment using this virus without Cre to check for any possible spurious expression. Surprisingly, there was considerable mCitrine but no HA expression. How is it possible that there is significant mCitrine expression without Cre? Why is mCitrine expressed and no HA? Does this mean the KORD itself is being expressed without the Cre recombinase too?
ReplyDeleteThanks for your help!