In vivo evidence for suppression of neuronal firing by hM4Di
I received an email last week asking if there was any evidence of this (other than readouts like cFos and bath application of CNO to slices).
There is a nice paper in Nature 2015 (Fig 4) using GCAMP as a readout, here in Nature Communications using power-spectrum EEG as a readout and in Neuron using single unit recordings in vivo.
There are >100 papers citing use of hM4Di for this purpose in vivo but most (not surprisingly) look at slices to validate silencing/suppression of activity.
There is a nice paper in Nature 2015 (Fig 4) using GCAMP as a readout, here in Nature Communications using power-spectrum EEG as a readout and in Neuron using single unit recordings in vivo.
There are >100 papers citing use of hM4Di for this purpose in vivo but most (not surprisingly) look at slices to validate silencing/suppression of activity.
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