In vivo evidence for suppression of neuronal firing by hM4Di

I received an email last week asking if there was any evidence of this (other than readouts like cFos and bath application of CNO to slices).

There is a nice paper in Nature 2015 (Fig 4) using GCAMP as  a readout, here in Nature Communications using power-spectrum EEG as  a readout and in Neuron using single unit recordings in vivo.

There are >100 papers citing use of hM4Di for this purpose in vivo but most (not surprisingly) look at slices to validate silencing/suppression of activity.

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