I am curious about the GsD DREADD mice. From the Genesis paper, I am not sure whether they are made or not. If so, would they will be available from JAX?
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
Hi Bryan,
ReplyDeleteI am curious about the GsD DREADD mice. From the Genesis paper, I am not sure whether they are made or not. If so, would they will be available from JAX?
Thanks,
Biao
They were made and need to be remade. Will update when re-deposited in JAX
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