CNO via minipump (Science paper, Moser labs)
I often get questions regarding CNO via minipump and here is a nice example where that seems to work.
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CNO in drinking water
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
How best to dissolve CNO ...it depends on what it is soluble in (and that depends on the particular polymorph)
I recently received the following question: " My question is with regard to the use of CNO in drinking water (in mouse studies). Your recipe calls for the addition of a small amount of DMSO to dissolve CNO at a concentration of 5 mg in 200 mL. As we and others routinely dissolve CNO in 0.9% saline (no DMSO) at concentrations up to four times this amount, I am curious whether the addition of DMSO to CNO solutions for H2O drinking is critical, or whether the properties of CNO permit it's direct dissolution in H2O at concentrations from 5-20 mg per 200 mL. There appears to be no difficulty dissolving such concentrations in H2O in my hands, but I would prefer to hear your perspective. Is there a published reference for the solubility profile of CNO in H2O or 0.9% saline?" It basically depends upon the source of the CNO. CNO via the NIH RAID program (which is what we use) is not readily soluble in H20 while commercial CNO is. Although we've verified that both source
It seems that the authors did not prepare fresh CNO on a daily basis (which would be difficult with a osmotic minipump), and I'm quite surprised that CNO remained stable for 7 days at body temperature (in a minipump). But definitely interesting method to try if you need to deliver CNO over prolonged period.
ReplyDeleteHas anyone else successfully kept CNO in solution for an extended period of time at body temperature? Does the solvent used (DMSO, DI Water, Saline, Ethanol) affect this?
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