CNO via minipump (Science paper, Moser labs)
I often get questions regarding CNO via minipump and here is a nice example where that seems to work.
CNO in drinking water
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
It seems that the authors did not prepare fresh CNO on a daily basis (which would be difficult with a osmotic minipump), and I'm quite surprised that CNO remained stable for 7 days at body temperature (in a minipump). But definitely interesting method to try if you need to deliver CNO over prolonged period.
ReplyDeleteHas anyone else successfully kept CNO in solution for an extended period of time at body temperature? Does the solvent used (DMSO, DI Water, Saline, Ethanol) affect this?
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