It seems that the authors did not prepare fresh CNO on a daily basis (which would be difficult with a osmotic minipump), and I'm quite surprised that CNO remained stable for 7 days at body temperature (in a minipump). But definitely interesting method to try if you need to deliver CNO over prolonged period.
Has anyone else successfully kept CNO in solution for an extended period of time at body temperature? Does the solvent used (DMSO, DI Water, Saline, Ethanol) affect this?
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
An interesting and provocative paper recently published in Nature Communications: " Modulation of anti-tumor immunity by the brain’s reward system " They conclude: " Activation of the reward system in tumor-bearing mice (Lewis lung carcinoma (LLC) and B16 melanoma) using chemogenetics (DREADDs), resulted in reduced tumor weight. This effect was mediated via the sympathetic nervous system (SNS), manifested by an attenuated noradrenergic input to a major immunological site, the bone marrow. Myeloid derived suppressor cells (MDSCs), which develop in the bone marrow, became less immunosuppressive following reward system activation. By depleting or adoptively transferring the MDSCs, we demonstrated that these cells are both necessary and sufficient to mediate reward system effects on tumor growth. Given the central role of the reward system in positive emotions, these findings introduce a physiological mechanism whereby the patient’s psychological state can impact anti-...
It seems that the authors did not prepare fresh CNO on a daily basis (which would be difficult with a osmotic minipump), and I'm quite surprised that CNO remained stable for 7 days at body temperature (in a minipump). But definitely interesting method to try if you need to deliver CNO over prolonged period.
ReplyDeleteHas anyone else successfully kept CNO in solution for an extended period of time at body temperature? Does the solvent used (DMSO, DI Water, Saline, Ethanol) affect this?
ReplyDelete