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How best to dissolve CNO ...it depends on what it is soluble in (and that depends on the particular polymorph)
I recently received the following question:
"My question is with regard to the use of CNO in drinking water (in mouse studies). Your recipe calls for the addition of a small amount of DMSO to dissolve CNO at a concentration of 5 mg in 200 mL. As we and others routinely dissolve CNO in 0.9% saline (no DMSO) at concentrations up to four times this amount, I am curious whether the addition of DMSO to CNO solutions for H2O drinking is critical, or whether the properties of CNO permit it's direct dissolution in H2O at concentrations from 5-20 mg per 200 mL. There appears to be no difficulty dissolving such concentrations in H2O in my hands, but I would prefer to hear your perspective. Is there a published reference for the solubility profile of CNO in H2O or 0.9% saline?"
It basically depends upon the source of the CNO. CNO via the NIH RAID program (which is what we use) is not readily soluble in H20 while commercial CNO is. Although we've verified that both sources are …
"My question is with regard to the use of CNO in drinking water (in mouse studies). Your recipe calls for the addition of a small amount of DMSO to dissolve CNO at a concentration of 5 mg in 200 mL. As we and others routinely dissolve CNO in 0.9% saline (no DMSO) at concentrations up to four times this amount, I am curious whether the addition of DMSO to CNO solutions for H2O drinking is critical, or whether the properties of CNO permit it's direct dissolution in H2O at concentrations from 5-20 mg per 200 mL. There appears to be no difficulty dissolving such concentrations in H2O in my hands, but I would prefer to hear your perspective. Is there a published reference for the solubility profile of CNO in H2O or 0.9% saline?"
It basically depends upon the source of the CNO. CNO via the NIH RAID program (which is what we use) is not readily soluble in H20 while commercial CNO is. Although we've verified that both sources are …
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ReplyDeleteI'm contemplating a DREADD experiment (my first!) in which I'd like to characterize the time course over which systemically injected CNO excites Cre-labeled neurons of mice in which AAV-FLEX hM3Dq has been stereotaxically injected into a heterogeneous region of the hypothalamus. I would like to prepare a slice at intervals after the CNO injection and determine the activity of neurons labeled with Cre-dependent TdTomato. My question is whether the CNO will have washed out by the time I prepare the slice and patch the cells? Has anyone tried this? Is it necessary to do in vivo recordings? I see no way to selectively record from just the Cre (fluroescent) neurons.
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