This is a blog to provide feedback and comments from users regarding chemogenetic technologies.
Comparison of opto- and chemogenetic technologies for modulating feeding response.  Data modified from http://www.ncbi.nlm.nih.gov/pubmed/21209617 and http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069789/

Comments

  1. i guess viral expression is evident after 2-3 weeks. How about the upper limit? Something like 2-3 months? Any input would be valuable.

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  2. There is a fairly large literature about this in mice as AAV is integrated into the genome. I highlight one paper which shows expression of cargo for at least 12 month (http://online.liebertpub.com/doi/abs/10.1089/10430349950018995 )

    The longest we've looked for DREADD expression is 3 months--still expressed at high levels (Urban and Roth, unpublished).

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  3. Thank you so much Bryan. Can I have a question regarding CNO effect? How long does it last in mice at 5mg/kg dose ( when injected IP) ? Something like two hours or does it last longer?

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  4. Please look at the Neuron paper where this was addressed. It is within several minutes (http://www.ncbi.nlm.nih.gov/pubmed/21415127)

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