Use of DREADDs to Dissect Metabolic Pathways
Nice review by Jurgen Wess here
G12/13 PREFERRING DREADD
In a recent Cell paper the authors provide an elegant approach to create a G12/13-preferring DREADD. This will have a huge impact!
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CNO in drinking water
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
Dissolving Salvinorin B for KORD experiments
From Krashes lab: We make SalB fresh the day of injection. We use pure DMSO to dissolve SalB at a 10mg/kg concentration. For a 30g mouse: 30uL of a 10ug/uLsolution It's a very small volume, we usually do SC injection
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