G12/13 PREFERRING DREADD
In a recent Cell paper the authors provide an elegant approach to create a G12/13-preferring DREADD. This will have a huge impact!
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CNO in drinking water
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
DREADDs cure cancer?
An interesting and provocative paper recently published in Nature Communications: " Modulation of anti-tumor immunity by the brain’s reward system " They conclude: " Activation of the reward system in tumor-bearing mice (Lewis lung carcinoma (LLC) and B16 melanoma) using chemogenetics (DREADDs), resulted in reduced tumor weight. This effect was mediated via the sympathetic nervous system (SNS), manifested by an attenuated noradrenergic input to a major immunological site, the bone marrow. Myeloid derived suppressor cells (MDSCs), which develop in the bone marrow, became less immunosuppressive following reward system activation. By depleting or adoptively transferring the MDSCs, we demonstrated that these cells are both necessary and sufficient to mediate reward system effects on tumor growth. Given the central role of the reward system in positive emotions, these findings introduce a physiological mechanism whereby the patient’s psychological state can impact anti-...
Hi,
ReplyDeleteWe have been ordering DREADDs from the UNC vector core but today while trying to order we found that the Roth lab viruses have disappeared from there? Is this a bug? OR is there a new place to order them from? Thanks a lot!
Best,
Arghya
Caroni Group,
Friedrich miescher Institute, CH
The viral core can make custom preps but you need to get the plasmid from ADDGENE and send it to them. Addgene will soon be providing AAV stocks as well.
DeleteThank you for the information!
DeleteBest,
Arghya
Dear Prof Roth,
DeleteOur group is also using DREADDs and we were wondering how soon will these be available as AAV stocks from ADDGENE?
Many thanks,
Sanziana,
University of Oxford
Hopefully soon. Check back often
DeleteDear Prof Roth,
ReplyDeleteMay I know after iv or ip CNO administration, what time does it block/activate Dreadd to maximum level and how long does it stay at the plateau.
Best regards
Irfan