CNO in drinking water
We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
Hi,
ReplyDeleteWe have been ordering DREADDs from the UNC vector core but today while trying to order we found that the Roth lab viruses have disappeared from there? Is this a bug? OR is there a new place to order them from? Thanks a lot!
Best,
Arghya
Caroni Group,
Friedrich miescher Institute, CH
The viral core can make custom preps but you need to get the plasmid from ADDGENE and send it to them. Addgene will soon be providing AAV stocks as well.
DeleteThank you for the information!
DeleteBest,
Arghya
Dear Prof Roth,
DeleteOur group is also using DREADDs and we were wondering how soon will these be available as AAV stocks from ADDGENE?
Many thanks,
Sanziana,
University of Oxford
Hopefully soon. Check back often
DeleteDear Prof Roth,
ReplyDeleteMay I know after iv or ip CNO administration, what time does it block/activate Dreadd to maximum level and how long does it stay at the plateau.
Best regards
Irfan