SCIENCE: DREADDs and Hypothalamic regulation of regionally distinct adult neural stem cells and neurogenesis
Interesting first-release Science paper here with direct CNO injections
Posts
Great use of multiplexing KORD, hM3Dq and hM4Di to interrogate histaminergic control of grooming
paper here in PNAS Cool videos as well here:
DREADDs: Lateral thalamic control of nociceptive response after whisker pad injection of varicella zoster virus
Thalamic neuronal activity was modulated after injecting an adeno-associated virus (AAV) expressing an engineered acetylcholine Gi-protein coupled receptor. This receptor inhibits neuronal firing when bound by clozapine -n- oxide (CNO). VGAT expression was attenuated in the thalamus by injecting an AAV construct that expressed a VGAT silencing shRNA. VZV induced nociception was significantly decreased after administering CNO in male rats. Nociception significantly increased concomitant with increased thalamic c-fos expression after attenuating thalamic VGAT expression. These data establish that the lateral thalamus (posterior, ventral posteromedial , ventral posterolateral and/or reticular thalamic nucleus) controls VZV induced nociception in the orofacial region, and that GABA in this region appears to reduce the response to VZV induced nociception possibly by gating facial pain Nice controls here:
Galanin-expressing GABA neurons in the lateral hypothalamus modulate food reward and non-compulsive locomotion
Really great set of controls here
CNO via minipump (Science paper, Moser labs)
I often get questions regarding CNO via minipump and here is a nice example where that seems to work.
DREADDs and OPTO modulating VTA terminals
Dopamine terminals from the ventral tegmental area gate intrinsic inhibition in the prefrontal cortex
DREADD-modulation of 5HT neurons causes perseverative responding
Activity patterns of serotonin neurons underlying cognitive flexibility
