Should I make up CNO fresh or use frozen stocks?

Question:  I have a quick question about preparation of CNO. CNO will be prepared as 0.1 mg/ml in 0.25%DMSO/0.9% saline, and I am wondering whether this solution can be stored in 4C for a long term or should be stored in -20C (or should be prepared each day before using). 

 Answer: We recommend making CNO fresh before each use.  

Comments

  1. UnknownApril 27, 2015 at 10:52 AM

    Hello! Just to clarify, Is it acceptable to prepare fresh daily injections from frozen stocks? We are interested in starting dosing at 0.3mg/kg for a 30g mouse. Preparing this injection dose from powder would generate a huge amount of waste that I would like to avoid.
    -Cheers!

    ReplyDelete
  2. zendudestApril 27, 2015 at 10:54 AM

    What I would recommend is to store small use-once aliquots after you've made up a stock. Just make the aliquots a size that is convenient for one or two mice.

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