DREADDs in rats redux

An elegant paper in Cell today demonstrating (again) that local infusions of CNO can modulate terminal release in rats.  Again, TH-Cre rats were used and both excitatory and inhibitory DREADDs were used to modulate norepinephrine release and thereby modify choice decisions.

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CNO in drinking water

We frequently get requests regarding protocols for putting CNO in the drinking water for long-term studies. Here is our protocol: 1. Dissolve the CNO in a small volume of DMSO 2. Diluted the dissolved CNO in drinking water (e.g., 5mg/200ml) 3. Give mice with CNO drinking water  and protect from light using foil-wrapped bottles 4. The mice will receive 5mg/kg/day CNO (assume that mice weight 30g and consume 6ml water per day) 5. Prepare fresh daily. 6. A small amount of saccharine in the drinking water will mask the slightly bitter taste of CNO
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