Heteromeric species

I frequently receive questions regarding the potential for DREADDs to form functional homo- and hetereomeric species.  There is a nice paper published some time ago indicating that at least in transfected cells any homomeric species are not of any significance in terms of signaling (e.g. a dimer between a DREADD and a native muscarinic receptor).

The broader question regarding potential hetereomeric species is unclear.  I note in passing that recent papers have reported failure to replicate D1/D2 hetereodimers and mGluR2/5-HT2A heterodimers suggesting that this field is still somewhat controversial.

Comments

  1. UnknownJanuary 6, 2015 at 4:22 PM

    sorry this may not be the appropriate location..

    my question is regarding dissolving CNO.
    is the concentration of CNO in saline important?

    for example, if i want to do 3mg/kg for a 500g rat, i would ideally only inject a total volume of about 5mL.
    following this, can i mix 1.5 mg/ 5mL (.3mg per mL)?
    supposing of course good no suspension, but CNO is dissolved.

    thanks!!

    ReplyDelete
  2. zendudestJanuary 7, 2015 at 6:36 AM

    Provided it is in solution that should be fine.

    ReplyDelete
  3. UnknownJanuary 7, 2015 at 4:36 PM

    thanks a lot!

    ReplyDelete

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